Venue: PACIFICO Yokohama Exhibition Hall A, B, C Seminar Room 1,2

Applications of NEBuilder Assembly: A Rapid, One-Day Workflow for Cell-Free Protein Expression
Co-hosted by: New England Biolas Japan Inc.

Chairperson
Yohei Hanazaki (New England Biolas Japan Inc.)

Speaker
Kohei Matsubara (New England Biolas Japan Inc.)

Detail

NEB provides NEBuilder Assembly, known as a seamless cloning method that does not depend on restriction enzymes, making it highly flexible. This method has several advantages, including the ability to clone ssDNA oligos directly and produce intact plasmids without nicks. Here, we focus on the latter benefit and present a new application workflow combining NEBuilder and its intact circular products with our phi29-XT RCA kit and NEBExpress Cell-free E. coli Protein Synthesis System. This workflow enables rapid protein expression and screening; the entire process, PCR of the target DNA to the protein expression can be completed in one day. Additionally, rapid and extensive protein screening is possible when also possible when combined with automation.

Writing the Future with New Enzymes | Twist Pioneers Next-Generation Library Preparation
Co-hosted by: Twist Bioscience

Speaker
Masanori Noguchi (Twist Bioscience)

Speaker
Toshiki Taya (Twist Bioscience)

Detail

Twist Bioscience is advancing the development of optimized next-generation sequencing workflows, in addition to offering capture panels that deliver uniform target enrichment. By creating a new enzyme, we’ve increased yields across challenging AT- and GC-rich regions, enabling more efficient generation of high-quality data. In this seminar, we will introduce a novel library preparation protocol powered by this proprietary enzyme.

Expanding the Possibilities of NGS Library Preparation from Single-Stranded DNA
Co-hosted by: Takara bio inc.

Speaker
Torii Shohei (TAKARA BIO INC.)

Detail

In next-generation sequencing (NGS) analysis, highly fragmented samples such as FFPE-derived DNA and ultra-short DNA fragments have posed significant challenges for conventional library preparation methods.
Takara Bio has long provided innovative solutions for low-input DNA analysis through technologies such as ThruPLEX and PicoPLEX. Building on this expertise, we have recently launched the ssDNA-Seq Kit, which incorporates a novel Single-Strand DNA Ligase capable of highly efficient ligation of single-stranded DNA molecules.
This kit enables simple and efficient NGS library preparation from both single-stranded and double-stranded DNA.
In this seminar, we will introduce the underlying principles and key features of this technology, along with application examples using challenging samples such as FFPE-derived DNA and cfDNA, which have traditionally been difficult to analyze with conventional methods.

Actranza® lab.: A New Pyro-drive Jet Injector for Needleless in vivo Research
Co-hosted by: DAICEL CORPORATION

Speaker
Micihinori Takashina (DAICEL CORPORATION)

Detail

In the fields of oligonucleotide therapeutics, gene therapy, vaccine development, and protein function analysis, there is still a major challenge: delivering target molecules into specific cells or tissues in vivo efficiently and minimally invasively. Standard needle syringe methods often place a burden on animals, show low reproducibility, and make precise delivery to target tissues difficult.
To address these issues, Daicel developed a research device called “Actranza® Lab.”, which applies the pyro-drive jet injector as a needleless technology. This device enables the accurate delivery of various molecules—such as DNA, mRNA, proteins, and peptides—through intradermal, subcutaneous, or intramuscular injection. It provides researchers with a new option for simple and minimally invasive administration that does not require adjuvants or LNPs.
In this seminar, we will introduce methods for gene delivery using “Actranza® Lab.” and share application examples in cancer and immunology research.
Please join this seminar to learn more about the new possibilities that this technology can bring to in vivo research.

Our fastest PCR reagent KOD One^®! Differences in the KOD series and how to use it
Co-hosted by: TOYOBO CO., LTD.

Speaker
Yuta Miyatake (TOYOBO CO., LTD.)

Detail

At TOYOBO, we offer KOD, a DNA polymerase known for its high accuracy and exceptional processivity. Leveraging the unique characteristics of this enzyme, we provide a diverse lineup of PCR reagents, including KOD One^®, which enables easy and rapid PCR.
In this seminar, we will introduce the features and differences of the KOD series, including our newly launched KOD FLEX^®. Through practical examples, we aim to share valuable information that will surely benefit your research.

The Importance of Nucleic Acid Purification and Quality Control in Next-Generation Sequencing
Co-hosted by: New England Biolas Japan Inc.

Chairperson
Kohei Matsubara (New England Biolas Japan Inc.)

Speaker
Yohei Hanazaki (New England Biolas Japan Inc.)

Detail

Nucleic acid purification is the most critical step that determines the success or failure of molecular biology experiments and next-generation sequencing (NGS).
In this seminar, we will cover from the basics to advanced applications of nucleic acid purification, specific QC (quality control) evaluation methods, and common pitfalls with practical solutions.
We will also introduce examples of what can happen if QC is neglected, and provide a comprehensive explanation of key points for obtaining reliable and reproducible data.

Writing the Future Without Limits｜Twist Leading Next-Gen Research with Oligo Pools
Co-hosted by: Twist Bioscience

Speaker
Masanori Noguchi (Twist Bioscience)

Speaker
Tomoko Sudo (Twist Bioscience)

Detail

Twist Bioscience’s unique DNA synthesis technology enables the precise, parallel synthesis of diverse sequences. Our flagship product, the Oligo Pools, achieves long-length synthesis up to 500 bp, allowing flexible incorporation of promoters, enhancers, guide RNAs, probes, and other essential elements. With outstanding uniformity and low error rates, Twist Oligo Pools help overcome challenges such as oversampling and experimental noise, leading to highly reproducible data even across long sequence space. In this seminar, we will introduce Twist’s high-quality Oligo Pools and highlight their applications through recent case studies.

SMART FCCS Fluorescently labeled particle measurement system
Co-hosted by: Cosmo Bio Co., Ltd.

Speaker
Akihiro Yamaguchi (Cosmo Bio Co., Ltd. Production Technology)

Detail

SMART FCCS is a system for measuring fluorescently labeled particles in solutions.
Concentration and size can be easily measured.
It can be used to measure lipid nanoparticles, proteins, and other particles.
It can also be used to evaluate antigen-antibody reactions.

The Potential of Lensless One-Chip Microscopic Observation Technology: Micro Imaging Device
Co-hosted by: IDDK Co., Ltd.

Speaker
Wataru Ikeda (IDDK Co., Ltd.)

Detail

IDDK is developing the Micro Imaging Device (MID), a semiconductor-based, lensless, one-chip microscopic observation system designed for broad application across research and industrial domains. Unlike conventional optical microscopes, MID employs a fundamentally distinct imaging principle, resulting in a system that is lightweight, compact, and highly amenable to digital control. These characteristics enable diverse modes of deployment, including automation, integration into equipment and facilities, parallelization and multiplexing, as well as continuous and in situ observation.
In this presentation, we highlight four key topics: 1) the development of a new flexible system adaptable to various research scenes, 2) the extension of functionality from bright-field to fluorescence imaging, 3) the implementation of hyperspectral illumination for label-free spectral property analysis, 4) the establishment of a fully automated bioexperimental platform for space using unmanned satellites.

Introduction to Human Frontier Science Program (HFSP)
Co-hosted by: Japan Agency for Medical Research and Development

Speaker
TBA (Japan Agency for Medical Research and Development)

Detail

The Human Frontier Science Program (HFSP) is a unique initiative established in 1989 to promote innovative international collaborative research in the life sciences. The program supports researchers as well as current and prospective PhD holders who are interested in the life sciences and wish to explore their ideas on a global scale. We will introduce the HFSP Research Grants Program for independent researchers and the HFSP Postdoctoral Fellowships Programs for early-career scientists.

Introducing the New LSM 990 Multimodal Confocal Laser Scanning Microscope.An innovative confocal laser scanning microscope integrated with the latest widefield 3D technology
Co-hosted by: Carl Zeiss Co., Ltd.

Speaker
Yasuhiko Sato (Carl Zeiss Co., Ltd.)

Detail

Zeiss introduces the new LSM990 confocal microscope, featuring Lightfield 4D imaging that redefines 3D imaging, the latest super-resolution imaging, ultra-multicolor imaging with over 13 colors, and molecular dynamics analysis—all in one comprehensive solution.

The world of art and science conveyed through microscopes
Co-hosted by: NIKON SOLUTIONS CO., LTD.

Speaker
Yui Imura (Nikon Solutions Co., Ltd.)

Detail

Would you like to experience the beauty depicted in the microscopic world and the hidden research possibilities it holds? The NIKON JOICO AWARD is a unique award that evaluates the artistic and academic merit of microscopic images. Launched in 2019, this year marks the seventh year. In this seminar, we will introduce past award-winning works and the research they carry out.